Application Cases

Among all electric field-based microfluidic pretreatment techniques, dielectrophoresis (DEP) offers advantages such as biocompatibility, label-free operation, controllability, and ease of integration. It holds significant potential for applications in the separation and detection of biological samples and has been utilized for the isolation and enrichment of various cell types, including white blood cells, red blood cells, cancer cells, circulating tumor cells, and cancer stem cells.

Aigtek’s power signal sources feature low distortion and high-stability output performance. Additionally, with built-in signal sources, they ensure more reliable microfluidic testing systems and simpler operation.

Experiment Name: Dielectrophoresis-Based Cell Sorting Test

Experimental Block Diagram:

Experimental Setup Image:

Experimental Procedure:
The microfluidic chip analysis system includes a sample processing module, a signal detection module, and a signal acquisition and data processing module. The sample processing module comprises a microfluidic fluorescence sensing chip integrated with DEP and magnetic bead enrichment dual pretreatment modes, a syringe pump, and a signal generator. Signal detection and acquisition are performed by a fluorescence testing device, and the captured fluorescence signals are processed by a computer. Polytetrafluoroethylene (PTFE) capillaries connect the syringe pump to the inlet and outlet of the microfluidic chip, forming a closed microfluidic delivery channel. Whole blood samples, buffer solutions, immunomagnetic beads, and fluorescent nanoprobes are introduced into the microfluidic chip via the syringe pump to drive the fluid flow. The DEP electrode lines on the microfluidic chip are connected to the signal generator to achieve blood cell separation and capture of target substances in the plasma. After blood cell separation and target capture are completed, the fluorescence testing device and its supporting software are activated to perform fluorescence signal detection, acquisition, and data processing, enabling rapid detection of cancer biomarkers.

Product Recommendation: ATG-2000 Series Power Signal Source

Figure: ATG-2000 Series Power Signal Source Specifications

Application Area: Sensor Technology

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