Application Cases

Single-cell sequencing, as an emerging technology, enables high-throughput sequencing at the single-cell level, helping to understand the complex and dynamic regulatory networks behind cell phenotypes and opening new avenues for studying the state of individual cells and intracellular activities.

The ATA-7000 series of high-voltage amplifiers from Antai Electronics can amplify voltage signals of arbitrary waveforms, meeting the testing requirements for different waveforms in experiments. With a maximum output voltage of up to 20 kVpp, they can satisfy the testing needs of the vast majority of microfluidic chips available on the market.

Experiment Name: Single-Cell Sequencing Based on Micro-Well Microfluidic Chips

Experiment Principle: Under an external electric field, cells experience varying degrees of polarization and align in the direction of the applied field. If an inhomogeneous electric field is applied, polarized particles will experience dielectrophoretic forces, which can be used to manipulate small cells.

Experiment Block Diagram:

Experiment Photographs:

Experiment Process: A control signal generator is used to adjust the output of the main signal generator, which is then connected to the input of the high-voltage amplifier. After amplification, the voltage signal is applied across the electrodes. Subsequently, a mixture of live and dead cells is introduced. By gradually increasing the voltage, it can be observed that live cells are significantly attracted by the electric field force. On the computer screen, the separation of live and dead cells in the channel can be observed.

Experiment Results: The microfluidic chip is used to separate live and dead cells. By continuously increasing the voltage of the main signal generator, the separation efficiency of the cells is calculated. As the voltage of the main signal generator increases, almost all live cells are attracted to the vicinity of the tip electrode when the voltage reaches 700 V, with a separation efficiency of 98%.

Application Directions: Biology, Medicine

Application Scenarios: Drug purification, drug toxicity testing, live/dead cell sorting, microfluidic chip structure testing

Product Recommendation: ATA-7000 Series High-Voltage Amplifiers

Figure: Specifications of the ATA-7000 Series High-Voltage Amplifiers