Application Cases

Immunomagnetic bead-based cell separation relies on the binding of cell surface antigens to specific monoclonal antibodies conjugated with magnetic beads. In an external magnetic field, cells bound to antibody-conjugated magnetic beads are retained and captured within the magnetic field, while cells lacking the corresponding surface antigen—unable to bind to the specific antibody-magnetic bead complex—do not exhibit magnetism and are not retained. This enables efficient cell separation. Immunomagnetic bead (IMB) technology for cell sorting is characterized by its high efficiency, speed, simplicity, non-toxicity, cost-effectiveness, high purity of separation, and preservation of cell viability. It has been widely applied in clinical diagnostics, as well as in the isolation and detection of various tumor cells, bone marrow cells, blood cells, bacteria, and other microorganisms.

Aigtek's power amplifiers are extensively used in electromagnetic applications, with a maximum output current of up to 40 Ap, capable of driving most coils on the market and meeting the requirements for immunomagnetic bead-based cell separation experiments.

Experiment Name: Immunomagnetic Bead-Based Cell Separation Experiment

Experimental Principle: The process of immunomagnetic bead-based cell separation typically involves two steps: first, the specific binding of immunomagnetic beads to cells, primarily driven by the antigen-antibody interaction; second, the separation of cells through the interaction of the antigen-antibody-magnetic bead complex with an external magnetic field.

Experimental Block Diagram:

Experimental Setup Image:

Experimental Procedure:
Two excitation signals with a 90° phase difference are generated by a signal generator and amplified using the ATA-320 power amplifier to ensure a current of 40 Ap is applied to the coils. The two sets of coils generate a uniform magnetic field within a cubic specimen, allowing for the study of cell movement trajectories.

Experimental Results:
For immunomagnetic beads used in cell separation, when the particle diameter is less than 30 nm, the beads exhibit superparamagnetism. This means they display strong magnetism in the presence of an external magnetic field but remain stably dispersed in solution without magnetism once the external field is removed. This property provides an excellent foundation for rapid and efficient cell separation. When the particle diameter exceeds 100 nm, separation can be achieved in a weak magnetic field, but the beads are prone to sedimentation and adsorb fewer biological molecules. Therefore, the size of the magnetic beads must be selected based on the type of target substance to be separated.

Role of the Power Amplifier:
In this experiment, the power amplifier provides a highly stable, low-distortion AC signal with a maximum output of 40 A_p to drive the coils and generate the required magnetic field.

Application Areas: Medical, Biological

Application Scenarios: Cell Separation

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